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    Please use this identifier to cite or link to this item: http://ccur.lib.ccu.edu.tw/handle/A095B0000Q/253


    Title: 奈米金光柵耦合生物感測器;Research of Label-free Grating Coupling Biosensor Modified with Gold Nanoparticles
    Authors: 高從華;KAO, TSUNG-HUA
    Contributors: 機械工程系研究所
    Keywords: 奈米金;光柵耦合生物感測器;Gold nanoparticle;Refractive index detection;Label-Free Biosensor
    Date: 2017
    Issue Date: 2019-07-17
    Publisher: 機械工程系研究所
    Abstract: 本研究使用光柵耦合生物感測器結合奈米金的修飾進行靈敏度檢測及生化檢測。晶片製程採用射出成型大量製作品質均一的光柵晶片,再運用濺鍍的方式製作晶片的二氧化鈦波導層,並藉由調整濺鍍含氧量及濺鍍時間來最佳化晶片的靈敏度,在濺鍍參數為含氧量40%、波導層厚度90 nm時,可使晶片之靈敏度最佳化,由小範圍折射率(n=1.3355至n=1.343)蔗糖溶液檢測靈敏度可達-35 RIU^-1。 本研究設計雙槽道光柵耦合生物感測器,為了檢測奈米金修飾在光柵及波導層平面的效應,在晶片修飾奈米金後進行小範圍折射率(n=1.3355至n=1.343)蔗糖溶液檢測靈敏度。光柵在修飾奈米金前(晶片靈敏度未最佳化),靈敏度為-18.34±0.87 RIU^-1 (n=3),修飾後靈敏度為-11.93±0.16 RIU^-1 (n=3)有下降的趨勢;在平面波導測試晶片靈敏度則提升,從未修飾的-0.12 ±0.38 RIU^-1提升到-1.47 ±0.22 RIU^-1。 最後對晶片進行生化檢測,未修飾之光柵晶片檢測可測得平均最小濃度(LOD)為1.11×10^-7±2.67×10^-8 g/ml (n=3),最佳LOD為8.08×10^-8 g/ml。在光柵上修飾奈米金後進行生化檢測(DNP/anti-DNP)的檢測可測得平均最小濃度(LOD)為6.05×10^-8 ±4.61×10^-9 g/ml (n=3),最佳LOD為5.54×10^-8 g/ml。奈米金有提升晶片生化檢測的效果。
    In this study, we immobilize the gold nanoparticles (AuNPs) on a label-free biosensor which uses grating waveguide couplers for sensitivity detection and immunoassay of dinitrophenyl/anti-dinitrophenyl antibody (DNP/anti-DNP). In terms of fabrication processes, the injection molding and sputtering are used, by adjusting the sputtering oxygen content and sputtering time to optimize the chip sensitivity. When the sputtering oxygen content is 40% and the thickness of the waveguide layer is 90 nm, the biosensor has the optimum sensitivity of -35 RIU^-1, when the refractive index of sucrose solution changes from 1.3355 to 1.343. This study develops a dual-channel grating coupled biosensor to investigate the effect of immobilized location (the grating or the waveguide) of AuNPs on the sensitivity. The sensitivity of the biosensor’s grating is -18.14 ±0.87 RIU^-1 (n=3), and the sensitivity after modified AuNPs is -11.93 ±0.16 RIU^-1 (n=3). After AuNPs are modified on the waveguide, the sensitivity of the biosensor improves from -0.12 ± 0.38 RIU^-1 (n=3) to -1.47 ± 0.22 RIU^-1 (n=3). Finally, this study tests the immunoassay of DNP/anti-DNP. The measured average limit of detection (LOD) of the unmodified biosensor is 1.11×10^-7 ±2.67×10^-8 g/ml (n=3), and the best LOD is 8.08×10^-8 through DNP/anti-DNP interaction;and the measured average LOD of the AuNPs modified biosensor is 6.05×10^-8±4.61×10^-9 g/ml (n=3) , and the best LOD is 5.54×10^-8 g/ml through DNP/anti-DNP interaction. This indicates that the AuNPs improves the performance of the biosensor in the immunoassay of DNP/anti-DNP.
    Appears in Collections:[機械工程學系] 學位論文

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