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    Please use this identifier to cite or link to this item: http://ccur.lib.ccu.edu.tw/handle/A095B0000Q/245


    Title: 酵素薄膜之潤濕梯度微接觸印刷製程可行性研究
    Authors: 陳威廷;CHEN, WEI-TING
    Contributors: 機械工程系研究所
    Keywords: 微接觸印刷;酵素;潤濕梯度;Enzyme;Micro contact printing;Wetting gradient
    Date: 2017
    Issue Date: 2019-07-17
    Publisher: 機械工程系研究所
    Abstract: 本論文研究的目的是希望能將微接觸印刷(micro-contact printing,μCP)技術做擴展,從目前的光電應用擴展到生物感測。本論文利用微接觸印刷的方法結合潤濕梯度的原理使類酵素溶液(沒有酵素,但流體行為類似酵素的溶液,由台欣生物科技研發股份有限公司提供)在塗佈時就能產生圖案化的薄膜,再將其轉印至所需要的基板上。這不只使微接觸印刷有更多的應用,也能減少酵素材料的浪費。本論文會對潤濕梯度圖案化工作原理做推導,並且解釋在不同情況下液體移動的情形。接著藉由計算液體體積去估測潤濕梯度圖案化結果的面積大小。由於圖案化薄膜很薄,易受揮發的影響,因此在提拉塗佈的部份也會將揮發的問題考慮進去,並且探討揮發對提拉塗佈的影響。 在圖案化上墨的實驗中會遇到圖案無法成膜、圖案變形與圖案大小不一的問題,經過提拉速度與環境濕度的控制後已經可以達到較穩定的塗佈。在圖案化薄膜轉印的實驗中可以成功將圖案化薄膜轉印至玻璃,但是在轉印至碳墨基板時會遇到碳墨基板不平的問題造成轉印困難。碳墨基板經過表面平坦化後有明顯改善,不過只能達到將部份薄膜轉印至碳墨基板的結果。目前此技術在聚二甲基矽氧烷(Polydimethylsiloxane,PDMS)印模上利用 UV Ozone Cleaner(UVO)處理所產生的潤濕梯度表面已經能夠成功塗佈直徑為 2.2 mm的圓形圖案化薄膜,並且成功的轉印至玻璃基板上,圖案的完整性也達到 90% 以上。中文關鍵字 : 潤濕梯度、酵素、微接觸印刷
    The purpose of this paper is to extend the microcontact printing technology from the optoelectronics to the biosensors. This paper uses microcontact printing technology combined with the principle of wetting gradient to produce patterned films from enzyme-like solution (no enzyme, but fluid behavior is similar to enzymes) and subsequently transfer the films to the substrate of interest. This not only extends application scope of the microcontact printing but also reduces the waste of expensive enzyme materials. This paper will derive the principle of wetting gradient patterning. And explains the movement of the liquid in different situations. The size of the patterned area is estimated by calculating the volume of the liquid. Since the patterned thin film is easily affected by evaporation. So it will take into account the evaporable issues, and explore the impact of evaporation on the dip coating. In the experiment will encounter the pattern can not be film, pattern deformation and pattern size of the problem. After pulling speed and environmental humidity control has been able to achieve a more stable coating. In the experiment of patterned film transfer, the patterned film can be successfully transferred to glass. But in the transfer to the carbon substrate will encounter the problem of uneven substrate lead to transfer failure. Surface of the carbon substrate after planarizing significantly improved. But only the part of the film transfer to the carbon substrate. At present, this technology has successfully coated a circular patterned films with a diameter of 2.2 mm which successfully been transferred onto a glass substrate. The area ratio between printing result and coating result has reached more than 90%.Keywords : Wetting gradient, Enzyme, Microcontact printing
    Appears in Collections:[機械工程學系] 學位論文

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