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    Please use this identifier to cite or link to this item: http://ccur.lib.ccu.edu.tw/handle/A095B0000Q/114


    Title: 微型癌細胞阻抗量測模組之研發;Development on the Miniaturized Impedance Measurement Module for Cancerous cells
    Authors: 葉日豪;YEH, JIH-HAO
    Contributors: 機械工程系研究所
    Keywords: 微型阻抗模組;DEPIM;導納阻抗;介電泳;循環腫瘤細胞;planar interdigitated microelectrodes;impedance measuring system;lung cancer;DEPIM
    Date: 2018
    Issue Date: 2019-05-23 12:52:35 (UTC+8)
    Publisher: 機械工程系研究所
    Abstract: 分析細胞數量與細胞型態提供了我們在早期判斷癌症的重要依據,並即時地監控療程進度。利用電性檢測的原理在近幾年成為非常熱門的技術。在文獻中透過阻抗量測來檢測癌細種類的技術已經備受關注。本文提出了一種利用介電泳阻抗量測(DEPIM)方法研究人肺細胞株的微流體晶片,圓形與直線形狀的指叉式微電極的微電極圖案是由微製程技術製作而成。分別將不同的細胞數樣本注射在次毫米體積的微腔室中,其中細胞不僅能透過介電泳增強其集中在微電極表面上的現象,更可從捕獲在微電極上的細胞測量出電阻抗特性,並更進一步地發現在每種細胞的導納值變化與細胞數之間存在著線性關係。藉由這種特性可以量測出人類癌細胞(A549)與正常細胞(MRC-5)之差異,進而達到癌細胞檢測之目的。
    Cancer has become a worldwide disease, and increases rapidly in recent years. A precise treatment depends on the accuracy of the diagnostic technique, in which includes analysis of cell number and cellular morphology. This study presented the development on the microfluidic device utilizing the dielectrophoretic impedance measurement (DEPIM) method which explores the human lung cell lines. The microchip consists of interdigitated microelectrodes with the circle -on-line shape and a micro chamber in which both DEP concentrating and impedance sensing for cells inside. This micro device has been fabricated by standard microfabrication processes. A highly sensitivity impedance measurement circuit board was built for the chip. It provides the sinusoidal signals with the frequencies in a wide range, so that the experiments can be performed in a reliable excitation. The results show a linear relationship between the admittance variation and the cell number in the different cell lines. The significant difference in the slope of these characteristic lines were adopted to distinguish two different lung cells, including A549- human lung carcinoma cells and MRC-5- human normal lung epithelial cells. The device is a simple-to-operate with high sensitivity, rapid response, inexpensive and portable application for cancerous cells.
    Appears in Collections:[機械工程學系] 學位論文

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