不同離子強度緩衝液下利用奈米間隙濃縮蛋白質之研究;Protein Preconcentration Utilizing Nano-insterstices between Self-Assembled gold nanoparticles under different Buffer concentrations

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Title:	不同離子強度緩衝液下利用奈米間隙濃縮蛋白質之研究;Protein Preconcentration Utilizing Nano-insterstices between Self-Assembled gold nanoparticles under different Buffer concentrations
Authors:	吳建陞;WU, CHIEN-SHENG
Contributors:	機械工程系研究所
Keywords:	濃縮;蛋白質;奈米通道;電雙層;自組裝薄膜;Preconcentration;Electric double layer;Protein;Nano channel;Self-assembled monolayer
Date:	2017
Issue Date:	2019-07-17
Publisher:	機械工程系研究所
Abstract:	人體內微量的蛋白質配合奈微米尺度的流道設計，可利用電壓的施加使的微米流道內產生蛋白質濃縮的現象，藉由預濃縮能夠提升蛋白免疫檢測的靈敏度，應用於癌症的早期診斷，且可更快速的對人類血液進行分析，並減少生物樣本的消耗；利用設計的濃縮晶片能夠將樣本蛋白經濃縮後藉由濃度的提高而增加免疫反應中抗原抗體鍵結反應的靈敏度。本研究設計並製造出能濃縮蛋白質之檢測微晶片，流道是以低成本的聚二甲基矽氧烷(Polydimethylsiloxane, PDMS)轉印經由黃光微影製程的晶片圖形，利用自組裝金奈米粒子(AuNPs)在施加電場時會產生奈米裂縫，這時會有濃度極化現象發生，離子損耗區會有電雙層的堆疊，堆疊的厚度與金奈米粒子一致，來進行蛋白質濃縮，本研究使用市售螢光胎牛血清蛋白來進行濃縮實驗，本研究成果顯示，探討不同的緩衝液的濃度下進行濃縮蛋白實驗，實驗結果顯示4 mM 濃度為較適當濃度進行蛋白質濃縮。 In this study, the preconcentration chip for proteins was fabricated using simple standard soft lithography with a polydimethylsiloxane (PDMS) replica.Surface modification with 3-aminopropyl-triethoxysilane (APTES) and of gold nanoparticles were done on the surface of the present chip. When an electric field was applied the preconcentration chip, it generated Nano-insterstices. Self-assembled gold nanoparticles (AuNPs) had the exclusion-enrichment effect at this time. Ion stacked thickness as the same as Self-assembled gold nanoparticles(AuNPs) in ion depletion region.At this moment, protein could be concentrated because it had enough electric double layer thickness.In this study, different Phosphate buffer concentration had different electric double layer thickness,According the experimental results, 4 mM concentration was suitable concentration.
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