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    Please use this identifier to cite or link to this item: http://ccur.lib.ccu.edu.tw/handle/A095B0000Q/169


    Title: 結合三明治法與光柵耦合生物感測器應用於綠膿桿菌藥物通道蛋白檢測
    Authors: 曾柏勳;ZENG, BO-XUN
    Contributors: 機械工程系研究所
    Keywords: 藥物通道蛋白;綠膿桿菌;光柵耦合生物感測器;三明治法;Drug channel protein;Pseudomonas;Grating coupling biosensor;Sandwich ELISA
    Date: 2018
    Issue Date: 2019-05-23 12:52:48 (UTC+8)
    Publisher: 機械工程系研究所
    Abstract: 本研究發展了一光柵耦合生物感測器並結合三明治法針對綠膿桿菌藥物通道蛋白(OprD)進行檢測。由於醫院現行對於綠膿桿菌抗藥性之鑑定方式需耗費冗長的時間,若以其他生物感測器測得其在低濃度時之抗藥反應,則須具備高靈敏度之性能,本研究之感測方式即具備高靈敏度及專一性之特性,藉由三明治法除了可增加結合反應的專一性及放大反應訊號外,同時可以改善光柵感測器漸逝波場深度及小分子待測物尺寸匹配問題,有效降低感測器之偵測極限。研究中亦針對晶片表面修飾分子種類及濃度進行探討,期望藉由較佳的修飾條件獲得穩定且高效率的感測晶片。除此之外,由於本研究之光柵耦合生物感測器是利用偵測光柵表面有效折射率做為檢測機制,屬於一開放式生物感測平台,藉由修飾不同的抗體分子即可進行多種待測物檢測,搭配三明治法更能提升其感測性能,因此相當具有發展為一臨床檢測系統之潛力。
    This study developed a grating-coupled biosensor and combined the sandwich ELISA to detect Pseudomonas aeruginosa drug channel protein (OprD).Because the hospital's current identification method for the resistance of Pseudomonas aeruginosa takes a long time, if it is measured by other biosensors at a low concentration, it must have high sensitivity performance. The sensing method of this study has the characteristics of high sensitivity and specificity. In addition to increasing the specificity of the immune response and amplifying the response signal by the sandwich ELISA. The evanescent wave field depth of the grating sensor and the small molecule test object size matching problem can be simultaneously improved and effectively improve the detection limit of the sensor. In the study, the types and concentrations of surface modification molecules on chips were also discussed. It is expected that stable and high-efficiency sensing chips can be obtained by better modification conditions. In addition, since the grating-coupled biosensor of this study utilizes the effective refractive index of the surface of the detection grating as a detection mechanism, it belongs to an open biosensing platform. By modifying different antibody molecules, a variety of analytes can be detected, and the sandwich ELISA can improve the sensing performance, so it has the potential to develop into a clinical detection system.
    Appears in Collections:[機械工程學系] 學位論文

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